■ 基本信息
別名: | TRC2-pLKO-Puro;pLKO.5-TRC2-Puro |
啟動(dòng)子: | U6 |
復(fù)制子: | pUC |
質(zhì)粒分類(lèi): | 病毒系列，慢病毒干擾載體 |
質(zhì)粒大小: | 7484bp |
原核抗性: | Amp |
真核抗性: | Puro |
克隆菌株: | Stbl3 |
培養(yǎng)條件: | 37度 |
表達(dá)宿主: | 哺乳細(xì)胞 |
誘導(dǎo)方式: | 無(wú)須誘導(dǎo)，瞬時(shí)表達(dá) |
5'測(cè)序引物:
| U6:ATGGACTATCATATGCTTACCGTA |
3'測(cè)序引物: | 根據(jù)序列設(shè)計(jì)引物 |
■ 質(zhì)粒屬性
質(zhì)粒宿主: | 哺乳細(xì)胞,慢病毒 |
質(zhì)粒用途: | 基因沉默 |
片段類(lèi)型: | shRNA
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片段物種: |
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原核抗性: | Amp |
真核抗性: | Puro
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熒光標(biāo)記: |
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■ 質(zhì)粒簡(jiǎn)介
The MISSION TRC2 Control Vector pLKO-puro is a lentivirus plasmid vector. This vector is in the TRC2 pLKO-puro plasmid backbone, which contains the WPRE. The vector does not contain an shRNA insert and is useful as a negative control in experiments using the TRC2 MISSION shRNA library clones. This allows one to examine the effect of transfection on gene expression and interpret the knockdown effect seen with shRNA clones.
